Author :- B Seneviratne, WMDR Fernando
Title :- Identification of Helicobacter pylori in Gastric Biopsies; Comparison of the R of the Rapid Urease Test with the Histological Giemsa Staining Technique

Helicobacter pylori (H. pylori) remains a prevalent chronic infection in the developing countries. H.pylori is an important agent associated with peptic ulcer disease, chronic gastritis and gastric malignancies. It can be detected by various invasive (rapid urease test and histology) and non-invasive tests (stool antigen test, urea breath test and serology). The aim of this study was to compare the performance of the Rapid Urease Test (RUT) with histological Giemsa staining technique. Data was collected from histopathology reports of antral biopsies, endoscopy and RUT reports of the patients (n=429) who underwent endoscopic examination at a tertiary care private Hospital from July 2013 to May 2014. The overall positivity for H. pylori was considered when either or both tests were positive. H.pylori were found in 183 (42.65%) patients by the RUT method and in 191(44.55%) patients with the Giemsa staining technique. Only 18 (4.19%) cases showed dis-concordant results between Giemsa staining and the RUT method. With 95% confidence interval, proportion of the dis-concordant result (18/429) of two identification techniques is not statistically significant (p-value=1.00). Sensitivity and specificity of the Giemsa staining method was 98.45% & 100% and the RUT was 88.14%, 94.89% respectively. Even though no statistical significance was found for dis-concordant results by both methods, substitution of the RUT test by the histopathology Giemsa staining technique should be considered with caution.

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 Published in: "Inculcating Professionalism for National Development "

 Date of Conference: 27, 28 August 2015

 Conference Location : PO BOX 32,Rathmalana, Sri Lanka
General Sir John Kotealwala Defence University


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